A Rapid and Sensitive Spectrophotometric Method for the Assay of Chymotrypsin
نویسندگان
چکیده
منابع مشابه
A rapid and sensitive spectrophotometric method for the assay of chymotrypsin.
For several years this laboratory has been concerned with an investigation of those enzymes in rat skin extracts which, in part at least, comprise the proteolytic enzyme system of this organ (l-5). In certain cases, methods have been proposed for the detection of their individual activities. However, certain contemplated studies, e.g. the possible physiological significance of these proteolytic...
متن کاملA Highly Sensitive Kinetic Spectrophotometric Method for the Determination of Ascorbic Acid in Pharmaceutical Samples
In this study, a new reaction system for quantitative determination of ascorbic acid was introduced. The developed method is based on inhibitory effect of ascorbic acid on the Orange G-bromate system. The change in absorbance was followed spectrophotometrically at 478 nm. The dependence of sensitivity on the reaction variables including reagents concentration, temperature and time was investiga...
متن کاملDevelopment and Validation of a Rapid Derivative Spectrophotometric Method for Determination of Tropicamide in Eye Drops
Tropicamide is an antimuscarinic agent used as eye drops for refractive examinations. The aim of this study was to develop a simple and suitable analytical method for determination of tropicamide in eye drops in the presence of excipients. A zero-crossing third and fourth derivative spectrophotometric method was described for determination of tropicamide in eye drops. The measurements wer...
متن کاملA Rapid and Sensitive Method for Determina-tion of Morphine in Saliva of Opium Addicts
Background: Saliva is an alternative biological matrix in drug testing. It has several advantages in comparison with other biological fluids such as blood and urine. Collection of saliva is simple, noninvasive and can be controlled under direct observation in drug abusers, or opium addicts. Methods: In the present study we report our experience with saliva collection, liquid-liquid extraction a...
متن کاملRapid, sensitive spectrophotometric method for quantitative determination of sulfatides.
A rapid, sensitive spectrophotometric method for the analysis of sulfatides is described. The assay method is based upon the formation of a colored complex between the cationic dye azure A and (anionic) sulfolipids; the complex is extractable by a solution of chloroform-methanol 1:1. With the exception of some of the phospholipids, the reaction is specific for the sulfolipids. Cardiolipin was t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1959
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)70291-3